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    Protocol

    Sealed droplet mount

    A single drop under a coverslip, sealed at the perimeter. The fastest way onto the stage when one condition is enough.

    Format
    Droplet mount
    Steps
    5
    Bench time
    ~5 min

    What you need.

    In the box

    • Vulcan slide

    You supply

    • Two-part silicone, A and B
    • 22 × 22 mm coverslip
    • Pipette
    Method

    Step by step.

    Step 1 of 5: Start with a clean slide
    01

    Start with a clean slide

    A Vulcan slide, logo end to the left, dry and free of dust.

    01 / 05
    Published

    As shown in.

    The mount above is the one this study built its samples on.

    Local thermodynamics govern formation and dissolution of Caenorhabditis elegans P granule condensates

    Fritsch et al.

    Reveals that protein-rich P granule condensates in C. elegans embryos form and dissolve reversibly with temperature, providing direct evidence that local thermodynamic principles spatially organize biomolecules in living cells.

    PNAS · 2021

    Studies used research-stage prototypes that preceded the commercial VTS-100.

    All publications
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